HE瓊脂干粉培養(yǎng)基配制方法
發(fā)布時(shí)間：
2022-12-27
作者：
日水生物
HE瓊脂干粉培養(yǎng)基配制方法: 稱取本品75.6g于1L蒸餾水或去離子水中/可按比例增加或減少配制量），加熱煮沸1分鐘使完全溶解，傾注平皿，備用。
Formula/配方： g/L
Peptone/蛋白胨：12.0g
Beef Extract/牛肉浸粉：3.0g
Sucrose/蔗糖：12.0g
Lactose/乳糖：12.0g
Salicin/水楊素： 2.0g
Bile Salts No.3/3號(hào)膽鹽：9.0g
Sodium Chloride/氯化鈉：5.0g
Sodium Thiosulfate/硫代硫酸鈉：5.0g
Ferric Ammonium Citrate/檸檬酸鐵銨：1.5g
Acid Fuchsin/酸性品紅： 0.1g
Bromthymol Blue/溴麝香草酚藍(lán)：0.065g
Agar /瓊脂：14.0g
pH7.5±0.1/25℃
Preparation method: the product is called 75.6g in 1L distilled water or deionized water / can increase or reduce the dosage in proportion), heat and boil for 1 minutes to completely dissolve, pour plate and spare.
Formula/ formula: g/L
Peptone/ peptone: 12.0g
Beef Extract/ beef dipping powder: 3.0g
Sucrose/ sucrose: 12.0g
Lactose/ lactose: 12.0g
Salicin/ salicin: 2.0g
Bile Salts No.3/3 bile salt: 9.0g
Sodium Chloride/ sodium chloride: 5.0g
Sodium Thiosulfate/ sodium thiosulfate: 5.0g
Ferric Ammonium Citrate/ ammonium citrate: 1.5g
Acid Fuchsin/ acid fuchsin: 0.1g
Bromthymol Blue/ bromothymol blue: 0.065g
Agar / agar: 14.0g
PH7.5 + 0.1/25 C