匹克氏肉湯基礎(chǔ)的配制[步驟方法]
發(fā)布時(shí)間：
2022-12-27
作者：
日水生物
匹克氏肉湯配制方法: 稱取本品30.0g于1L蒸餾水或去離子水中可按比例增加或減少配制量），微溫溶解，分裝,121℃高壓滅菌15分鐘,冷至50～55℃，無菌操作每50ml培養(yǎng)基基礎(chǔ)中添加1支20902(1:800三氮化鈉溶液)和2.5ml無菌脫纖維羊血,輕輕混勻備用。
Formula(匹克氏肉湯配方)： g/L
Tryptone 胰蛋白胨：10.0g
Heart Extract Powder牛心浸粉：20.0g
Crystal Violet結(jié)晶紫：0.002g
pH7.4±0.1(25℃
Preparation method: 30.0g in 1L distilled water or deionized water can increase or reduce the amount of preparation according to proportion), micro temperature dissolving, separation, 121 centigrade sterilization for 15 minutes, cold to 50~55 degrees, aseptic operation to add 1 20902 (1:800 three nitridation solution) and 2.5ml aseptic defibrous sheep blood for every 50ml culture foundation, and gently mix and spare .
Formula (formula):g/L
Tryptone tryptone: 10.0g
Heart Extract Powder cow heart dipping powder: 20.0g
Crystal Violet crystal violet: 0.002g
PH7.4 + 0.1 (25 C)