四號(hào)瓊脂基礎(chǔ)使用方法
發(fā)布時(shí)間:
2022-12-27
作者:
日水生物
四號(hào)瓊脂基礎(chǔ)配制方法:
稱取本品53.5g于1L蒸餾水或去離子水中(可按比例增加或減少配制量),攪拌煮沸溶解1分鐘,冷卻至50—55℃,每100ml培養(yǎng)基中添加2支20518(0.05%亞碲酸鉀)或0.5支20803(亞碲酸鉀溶液),搖勻,傾注平板備用。
Formula(四號(hào)瓊脂配方): g/L
Peptone(蛋白胨) 20.0g
Beef Extract(牛肉浸粉) 5.0g
Sodium Chloride(氯化鈉) 5.0g
Sodium Sulfite(無(wú)水亞硫酸鈉) 3.0g
Sodium lauryl sulfate(十二烷基硫酸鈉) 0.5g
Pig Bile Powder(豬膽粉) 5.0g
Rivanol(利凡諾) 0.03g
Gentamicin(慶大霉素) 500U
Agar(瓊脂) 15.0g
pH8.5±0.2(25℃)
Preparation method:
According to take this product 53.5 g in 1 l of distilled water or deionized water (can be in proportion to increase or decrease preparation), stir boiling dissolved 1 minute, cooled to 50 to 55 ℃, 100 per 100 ml medium to add two (0.05% potassium tellurite) or 0.5 20803 (potassium tellurite solution), shake well and pour into plate and set aside.
Formula: g/L
Peptone (Peptone) 20.0g
Beef Extract 5.0g
Sodium Chloride (nacl) 5.0 g
Sodium Sulfite 3.0g
Sodium lauryl sulfate (Sodium dodecyl sulfate) was 0.5g
Pig Bile Powder 5.0g
Rivanol 0.03g
Gentamicin 500U
Agar (Agar) 15.0g
PH8.5 + / - 0.2 (25 ℃)