Bolton 肉湯基礎(chǔ)配方
發(fā)布時(shí)間：
2022-12-27
作者：
日水生物
Bolton 肉湯基礎(chǔ)配方
配制方法:
稱取本品2.76g于100ml蒸餾水或去離子水中，121℃保高壓滅菌15分鐘,冷卻至45℃,添加1支20701（Bolton肉湯基礎(chǔ)添加劑）5ml無(wú)菌裂解脫纖維羊血,輕輕混勻,備用。
Formula(配方)： g/L
Peptic Digest of Animal Tissue
（動(dòng)物組織酶解物） 10.0g
Milk protein hydrolysate
（乳蛋白水解物) 5.0g
Yeast Extract（酵母浸粉） 5.0g
Sodium Chloride（氯化鈉） 5.0g
Sodium pyruvate（丙酮酸鈉） 0.5g
Sodium metabisulfite（偏重亞硫酸氫鈉）0.5g
Sodium carbonate（碳酸鈉） 0.6g
Alpha ketoglutaric acid（α-酮戊二酸） 1.0g
pH7.2±0.2 (25℃)
Bolton meat soup basic formula
Preparation method:
Said the 2.76 g amount of this product in 100 ml of distilled water or deionized water, 121 ℃ high pressure sterilization for 15 minutes, cooling to 45 ℃, add 1-20701 (Bolton broth base additives) 5 ml sterile sheep blood, crack free fiber blending gently, set aside.
Formula: g/L
Peptic Digest of Animal Tissue
(enzymolysis of animal tissue) 10.0g
Milk protein hydrolysate
(milk protein hydrolysate) 5.0g
Calcium Extract (Yeast Extract) 5.0g
Sodium Chloride (nacl) 5.0 g
Sodium pyruvate (Sodium pyruvate) is 0.5g
Sodium metabisulfite (Sodium bisulfite) 0.5g
Sodium carbonate (Sodium carbonate) 0.6g
Alpha ketoglutaric acid (prun-ketoglutaric acid) 1.0g
PH7.2 + / - 0.2 (25 ℃)