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察氏瓊脂培養(yǎng)基配方|配制說明

發(fā)布時間:

2022-12-27

作者:

日水生物


 察氏瓊脂培養(yǎng)基配制方法:

稱取本品55.0g于1L蒸餾水或去離子水中,加熱煮沸至完全溶解,分裝,121℃高壓滅菌20分鐘,滅菌結(jié)束后請搖勻,以防瓊脂沉積于器皿底部而凝固,備用。
 
Formula( 察氏瓊脂培養(yǎng)基配方): g/L
Sodium Nitrate(硝酸鈉)             3.0g
Dipotassium phosphate 
(磷酸氫二鉀)                                1.0g
Magnesium Sulfate(硫酸鎂)          0.5g
Potassium Chloride(氯化鉀)         0.5g
Ferrous Sulfate(硫酸亞鐵)      0.01g
Sucrose(蔗糖)                                30.0g
Agar(瓊脂)                                        20.0g
 pH7.3±0.2(25℃)
 

 The preparation method of qishi AGAR medium:

 
According to take this product 55.0 g in 1 l of distilled water or deionized water, heat to boil until completely dissolved, repackaging, 121 ℃ high pressure sterilization for 20 minutes, please shake after sterilization, to prevent AGAR deposited on the bottom of the vessel and solidification, and set aside.
 
 
Formula: g/L
Sodium Nitrate on 3.0g
Dipotassium phosphate
(dipotassium hydrogen phosphate) 1.0g
Magnesium Sulfate, Magnesium Sulfate) 0.5 g
Potassium Chloride (KCL) 0.5 g
Ferrous Sulfate (Ferrous Sulfate) 0.01g
Sucrose (Sucrose) 30.0g
Agar(Agar) 20.0g
PH7.3 + / - 0.2 (25 ℃)