高鹽察氏培養(yǎng)基配制
發(fā)布時間：
2022-12-27
作者：
日水生物
配制方法和配制過程:稱取本品110.0g于1L蒸餾水或去離子水中，加熱煮沸至完全溶解，分裝,121℃高壓滅菌20分鐘,滅菌結(jié)束后請搖勻,以防瓊脂沉積于器皿底部而凝固，備用。
Formula(配方)： g/L
Sodium Nitrate（硝酸鈉）2.0g
Dipotassium phosphate1.0g
（磷酸氫二鉀）
Magnesium Sulfate（硫酸鎂）0.5g
Potassium Chloride（氯化鉀）0.5g
Ferrous Sulfate（硫酸亞鐵）0.01g
Sodium Chloride（氯化鈉）60.0g
Sucrose（蔗糖) 30.0g
Agar(瓊脂) 16.0g
pH6.0±0.2(25℃)
Preparation method:
According to take this product 110.0 g in 1 l of distilled water or deionized water, heat to boil until completely dissolved, repackaging, 121 ℃ high pressure sterilization for 20 minutes, please shake after sterilization, to prevent AGAR deposited on the bottom of the vessel and solidification, and set aside.
Formula: g/L
Sodium Nitrate 2.0g
Dipotassium phosphate 1.0 g
(dipotassium hydrogen phosphate)
Magnesium Sulfate, Magnesium Sulfate) 0.5 g
Potassium Chloride (KCL) 0.5 g
Ferrous Sulfate (Ferrous Sulfate) 0.01g
Sodium Chloride (nacl) 60.0 g
Sucrose (Sucrose) 30.0g
Agar(Agar) 16.0g
PH6.0 + / - 0.2 (25 ℃)