1%吐溫 80-玉米瓊脂培養(yǎng)基基礎(chǔ)培養(yǎng)基配方
發(fā)布時(shí)間：
2022-12-27
作者：
日水生物
1%吐溫 80-玉米瓊脂培養(yǎng)基基礎(chǔ)培養(yǎng)基配方配制方法:
稱取本品21.0g于1L蒸餾水或去離子水中，加入10ml吐溫80（20204），加熱攪拌煮沸至完全溶解，分裝，121℃高壓滅菌15分鐘,滅菌結(jié)束后請(qǐng)搖勻，以防瓊脂沉積于器皿底部而凝固，備用。
1%吐溫 80-玉米瓊脂培養(yǎng)基基礎(chǔ)培養(yǎng)基Formula(配方)： g/L
Corn Steep Powder（玉米浸粉） 6.0g
Agar（瓊脂) 15.0g
1% twain 80- corn AGAR medium basic medium formula preparation method:
According to take this product 21.0 g in 1 l of distilled water or deionized water and add 10 ml polysorbate 80 (20204), heating boiling stir until completely dissolved, repackaging, 121 ℃ high pressure sterilization for 15 minutes, please shake after sterilization, to prevent AGAR deposited on the bottom of the vessel and solidification, and set aside.
Formula 1: g/L tween 80- corn AGAR medium basic medium Formula
Corn Steep Powder: 6.0g
Agar (Agar) 15.0g