減少PDA斜面培養(yǎng)基冷凝水的辦法有哪些？
發(fā)布時(shí)間：
2022-11-16
作者：
摘要：在我們做斜面培養(yǎng)基的時(shí)候，試管壁會出現(xiàn)一些凝結(jié)水。面對這樣的困擾，我們應(yīng)該怎么去實(shí)際解決一下呢？
PDA斜面培養(yǎng)基有冷凝水出現(xiàn)這根周圍的溫度有很大的關(guān)系。氣態(tài)水（水蒸氣）遇到冷空氣，到達(dá)結(jié)露點(diǎn)會變成液態(tài)水的過程，溫度越低冷凝就越快，冷凝水也就越多。如此，我們就能很好的預(yù)防和減少PDA斜面培養(yǎng)基冷凝水的產(chǎn)生了。
方法：縮小擺斜面后試管內(nèi)部和外部的溫差。
第一，適當(dāng)增加烘干棉塞的時(shí)間：在不影響擺斜面的情況下，增加烘干棉塞的時(shí)間，降低試管出鍋擺斜面時(shí)的溫度，縮小試管內(nèi)部和試管外部周圍的溫差。
第二，提高室溫。同樣起到縮小試管內(nèi)部和試管外部環(huán)溫的差異。
第三，擺斜面時(shí)將試管摞起來，呈梯形，最后在上面覆蓋干凈的紗布保溫。
最后，PDA斜面培養(yǎng)基制作時(shí)不可能完全避免溫差的存在，也就不可能完全避免冷凝水的產(chǎn)生，我們只能盡量減少PDA斜面培養(yǎng)基冷凝水的產(chǎn)生。
來自：青島日水生物培養(yǎng)基技術(shù)支持小組
There is a great relationship between the temperature of the PDA slope medium and condensed water. Gaseous water (water vapor) encounters cold air, the dew point will be reached into liquid water process, the lower the temperature condensation is faster, the more condensate. In this way, we can prevent and reduce the condensation of PDA inclined media.
Methods: the temperature difference between inside and outside of the test tube was reduced.
First, the time to dry the cotton plug is appropriately increased: in the case of not affecting the inclined plane, the time of drying the cotton plug is increased, the temperature of the test tube is lowered and the temperature difference between the inside of the test tube and the outside of the test tube is reduced.
Second, increase the room temperature. It also reduces the difference in temperature between inside and outside of test tube.
Third, when the inclined plane is placed, the test tube is stacked up to be trapezoid and finally covered with clean gauze for thermal insulation.
Finally, it is impossible to avoid the existence of the temperature difference completely when the PDA slope culture medium is made, and it is impossible to avoid the production of condensate completely. We can only minimize the production of condensate water in the PDA slope culture medium.