SOC、TB、YPD培養(yǎng)基的配制配方粉劑
發(fā)布時間：
2022-12-27
作者：
無菌的預(yù)制平板培養(yǎng)基
SOC、TB、YPD培養(yǎng)基的配制配方粉劑
成分、方法同SOB培養(yǎng)基的配制，只是在培養(yǎng)基冷卻到室溫后，除了在每100ml的小份中加1ml滅過菌的1mol/L氯化鎂外，再加2ml滅菌的1mol/L葡萄糖(18g葡萄糖溶于足夠水中，再用水補足到100ml，用0.22um的濾膜過濾除菌)。
日水soc培養(yǎng)基粉劑圖
將下列組分溶解在0.9L水中：
蛋白胨12g
酵母提取物24g
甘油4ml
各組分溶解后高壓滅菌。冷卻到60℃，再加100ml滅菌的170mmol/L KH2PO4/0.72 mol/L K2HPO4的溶液(2.31g的KH2PO4和12.54gK2HPO4溶在足量的水中，使終體積為100ml。高壓滅菌或用0.22um的濾膜過濾除菌)。
2&timeYT培養(yǎng)基
將下列組分溶解在0.9L水中：
蛋白胨16g
酵母提取物10g
氯化鈉4ml
如果需要用1N NaOH(～1ml)調(diào)整pH至7.0，再補足水至1L。注：瓊脂平板需添加瓊脂粉12g/L,上層瓊脂平板添加瓊脂粉7g/L。
YPD培養(yǎng)基
將下列組分溶解在0.9L水中：
蛋白胨20g
酵母提取物10g
葡萄糖20g
用水補足體積為1L后，高壓滅菌。建議在高壓滅菌之前，對色氨酸營養(yǎng)缺陷型每升培養(yǎng)基添加1.6g色氨酸，因為YPD培養(yǎng)基是色氨酸限制型培養(yǎng)基。為了配制平板，需要在高壓滅菌前加入20g瓊脂粉。
The preparation of SOC, TB, YPD medium.
SOC medium
Composition, the configuration of the method with an SOB medium, only in the culture medium after cooling to room temperature, in addition to each add 1 ml to 100 ml small portion out 1 mol/L magnesium chloride have bacteria, plus 2 ml sterilization of 1 mol/L glucose (18 g glucose in enough water, and then with water up to 100 ml, with 0.22 um membrane filter in addition to bacteria).
TB culture medium
Dissolve the following components in 0.9l water:
Peptone 12 g
Yeast extract 24g.
Glycerin 4 ml
High pressure sterilization after dissolution of each component. Cooling to 60 ℃, add 100 ml of sterilization tendency for 170 / L KH2PO4/0.72 mol/L K2HPO4 solution (2.31 2.31 g of KH2PO4 and gK2HPO4 dissolve in plenty of water, make the final volume of 100 ml. High pressure sterilization or using 0.22um filter membrane to remove bacteria.
2 & timeYT medium
Dissolve the following components in 0.9l water:
Peptone 16 g
Yeast extract 10g.
Sodium chloride 4 ml
If you need to adjust the pH to 7.0 with 1N NaOH(~ 1ml), then fill the water to 1L. Note: AGAR plate should be added with agar-agar powder 12g/L, and agar-agar plate with AGAR powder 7g/L.
YPD medium
Dissolve the following components in 0.9l water:
Peptone 20 g
Yeast extract 10g.
Glucose, 20 g
After using water for 1L, high pressure sterilization. It is suggested to add 1.6g tryptophan to tryptophan nutrient deficiency before the high pressure sterilization because the YPD medium is a tryptophan restriction medium. To prepare the plate, 20g AGAR powder is added before the high pressure sterilization.