胰蛋白示磷酸鹽肉湯的配方說明
發(fā)布時(shí)間：
2022-12-27
作者：
青島日水培養(yǎng)基公司
胰蛋白示磷酸鹽肉湯配制方法：稱取本品29.5g于1L蒸餾水或去離子水中[如果有需求可加入0.1-0.2%的瓊脂粉]，加熱煮沸至完全溶解，分裝，121℃高壓滅菌15分鐘,備用。
Tryptose Phosphate Broth Formula[配方]： g/L
Tryptone [胰蛋白示]：20.0g
Dextrose[葡萄糖]：2.0g
Sodium Chloride[氯化鈉] ：5.0g
Disodium Hydrogen Phosphate [磷酸氫二鈉]：2.5g
pH7.3±0.2 [25℃]
Trypin broth preparation method: called 29.5G in 1L distilled water or deionized water [if there is a need to add 0.1-0.2% agar powder], heat and boil to completely dissolve, separate, 121 centigrade high pressure sterilization for 15 minutes, spare.
Tryptose Phosphate Broth Formula[formula]:g/L
Tryptone [trypsin]:20.0g]
Dextrose[glucose]:2.0g
Sodium Chloride[sodium chloride]:5.0g
Disodium Hydrogen Phosphate [Disodium Hydrophosphate]: 2.5G
PH7.3 + 0.2 [25 C]