結(jié)晶紫中性紅膽鹽瓊脂含葡萄糖和乳糖的制備方法
發(fā)布時間：
2022-12-27
作者：
日水生物
結(jié)晶紫中性紅膽鹽瓊脂（含葡萄糖和乳糖）配制方法: 稱取本品51.5g于1L蒸餾水或去離子水中/可按比例增加或減少配置量),攪拌煮沸溶解1分鐘，冷卻至50-55℃傾注平板。
Formula/配方： g/L
Peptone/蛋白胨：7.0g
Dextrose /葡萄糖：10.0g
Lactose /乳糖：10.0g
Yeast Extract/酵母浸粉：3.0g
Sodium Chloride/氯化鈉：5.0g
Bile Salts No.3/3號膽鹽：1.5g
Neutral Red/中性紅：0.03g
Crystal Violet/結(jié)晶紫：0.002g
Agar/瓊脂：15.0g
pH7.4±0.2 /25℃)
Crystal violet neutral red bile salt agar (including glucose and lactose) preparation method: This product is called 51.5g in 1L distilled water or deionized water / can be proportionally increased or reduced configuration), stirred boiling to dissolve for 1 minutes, cooling to 50-55 centigrade to pour plate.
Formula: g/L
Peptone: 7.0g
Glucose: 10.0g
Lactose: 10.0g
Yeast extract: 3.0g
Sodium chloride: 5.0g
No. 3 bile salt: 1.5g
Neutral red: 0.03g
Crystal violet: 0.002g
Agar: 15.0g
PH7.4 + 0.2 /25 C