肉湯培養(yǎng)基I四硫磺酸鹽膽鹽亮綠肉湯的配置
發(fā)布時(shí)間：
2022-12-27
作者：
青島日水生物
肉湯培養(yǎng)基 I四硫磺酸鹽膽鹽亮綠肉湯的配置配制方法:稱取本品63g于1L蒸餾水或去離子水中（可按比例增加或減少配制量），加熱至沸騰即可，不可過分加熱，冷至25℃，混勻，分裝，備用。
配方： g/L
蛋白胨：8.6g
牛膽鹽：8.0g
氯化鈉：6.4g
碳酸鈣：20.0g
四硫磺酸鉀：20.0g
煌綠：0.07g
pH7.0±0.2(25℃)
Broth culture medium I four sulphur salt bile salt bright green broth collocation preparation method: called this product 63g in the 1L distilled water or deionized water (can increase or reduce the amount of preparation), heated to boiling, can not be overheated, cold to 25 degrees, mixing, packing, spare.
Formula: g/L
Peptone: 8.6g
Bovine bile salt: 8.0g
Sodium chloride: 6.4g
Calcium carbonate: 20.0g
Potassium sulphonate four: 20.0g
Brilliant green: 0.07g
PH7.0 + 0.2 (25 C)