亮綠酚紅瓊脂的配置方法說明
發(fā)布時間：
2022-12-27
作者：
日水生物
亮綠酚紅瓊脂干粉粉劑的配制方法:稱取本品54.75g于1L蒸餾水或去離子水中（可按比例增加或減少配制量），微溫溶解,分裝，121℃高壓滅菌15分鐘,滅菌結(jié)束后請搖勻，以防瓊脂沉積于器皿底部而凝固，備用。
配方)： g/L
蛋白胨：10.0g
酵母浸粉：3.0g
牛肉浸粉：5.0g
磷酸氫二鈉：1.0g
磷酸二氫鈉：0.6g
乳糖：10.0g
蔗糖：10.0g
酚紅：0.09g
煌綠：0.0125g
瓊脂：15.0g
pH6.9±0.2(25℃)
The preparation method of bright green phenol red agar dry powder powder: call this product 54.75g in 1L distilled water or deionized water (can increase or reduce the dosage in proportion), micro temperature dissolving, sub loading, 121 centigrade sterilization for 15 minutes, after sterilization, please shake well in order to prevent agar from depositing at the bottom of the utensils and solidify. Reserve.
Formula):g/L
Peptone: 10.0g
Yeast extract: 3.0g
Beef dipping: 5.0g
Two sodium hydrogen phosphate: 1.0g
Sodium dihydrogen phosphate: 0.6g
Lactose: 10.0g
Sucrose: 10.0g
Phenol red: 0.09g
Brilliant green: 0.0125g
Agar: 15.0g
PH6.9 + 0.2 (25 C)