亮綠瓊脂培養(yǎng)基的配方成分說明
發(fā)布時間：
2022-12-27
作者：
日水生物
亮綠瓊脂培養(yǎng)基干粉的配制方法:稱取本品58.0g于1L蒸餾水或去離子水中（可按比例增加或減少配制量），加熱煮沸至完全溶解，分裝，121℃高壓滅菌15分鐘,滅菌結束后請搖勻，以防瓊脂沉積于器皿底部而凝固，備用。
配方)： g/L
酵母浸粉：3.0g
胰酪蛋白胨：5.0g
動物組織胃蛋白酶消化物：5.0g
乳糖：10.0g
蔗糖：10.0g
氯化鈉：5.0g
苯酚紅：0.08g
煌綠：0.0125g
瓊脂：20.0g
pH6.9±0.2（25℃）
The preparation method of bright green agar medium dry powder: call this product 58.0g in 1L distilled water or deionized water (can increase or reduce the dosage in proportion), heat and boil to completely dissolve, pack, sterilize at 121 centigrade for 15 minutes, after sterilization, please shake well in order to prevent agar from depositing at the bottom of the utensils.
Formula):g/L
Yeast extract: 3.0g
Casein peptone: 5.0g
Pepsin digestibility in animal tissue: 5.0g
Lactose: 10.0g
Sucrose: 10.0g
Sodium chloride: 5.0g
Phenol red: 0.08g
Brilliant green: 0.0125g
Agar: 20.0g
PH6.9 + 0.2 (25 C)