配置SOC培養(yǎng)基制備方法
發(fā)布時間：
2022-12-27
作者：
日水生物
山東日水SOC培養(yǎng)基配制方法: 稱取本品31.4g于1L蒸餾水或去離子水中,加熱煮沸至完全溶解,分裝,116℃高壓滅菌30分鐘,備用。
Formula(配方)： g/L
Tryptone/胰蛋白胨：20.0g
Yeast Extract/酵母浸粉：5.0g
Sodium Chloride/氯化鈉：0.5g
Potassium Chloride/氯化鉀：0.186g
Magnesium Chloride/氯化鎂：0.95g
Magnesium Sulfate/硫酸鎂：1.2g
Dextrose/葡萄糖：3.6g
pH7.0±0.2 (25℃）
Shandong daily water SOC medium preparation method: This product is called 31.4g in 1L distilled water or deionized water, heated and boiled to complete dissolving, packing, sterilizing at 116 C for 30 minutes.
Formula (formula):g/L
Tryptone: 20.0g
Yeast extract: 5.0g
Sodium chloride: 0.5g
Potassium chloride: 0.186g
Magnesium chloride: 0.95g
Magnesium Sulfate: 1.2g
Glucose: 3.6g
PH7.0 + 0.2 (25 C)