葡萄糖銨瓊脂培養(yǎng)基的配制方法說(shuō)明
發(fā)布時(shí)間：
2022-12-27
作者：
日水生物
葡萄糖銨瓊脂培養(yǎng)基配制方法:
稱取本品24.2g于1L蒸餾水或去離子水中（可按比例增加或減少配制量)，加熱煮沸至完全溶解，分裝試管，121℃高壓滅菌15分鐘,備用。
Formula(葡萄糖銨瓊脂培養(yǎng)基配方)： g/L
Sodium Chloride 氯化鈉 5.0g
Magnesium Sulfate 硫酸鎂 0.2g
Ammonium Biphosphate 磷酸二氫銨 1.0g
Dipotassium Phosphate 磷酸氫二鉀 1.0g
Dextrose 葡萄糖 2.0g
Bromthymol Blue 溴麝香草酚藍(lán) 0.08g
Agar 瓊脂 15.0g
pH6.8±0.2(25℃)
Preparation method:
This product is called 24.2g in 1L distilled water or deionized water
(can be increased or reduced in proportion), heated to completely dissolved, packed in test tube, sterilized at 121 C for 15 minutes, standby.
Formula (formula):g/L
Sodium Chloride (sodium chloride 5.0g)
Magnesium Sulfate (Magnesium Sulfate 0.2g)
Ammonium Biphosphate
(two ammonium hydrogen phosphate 1.0g)
Dipotassium Phosphate (two potassium hydrogen phosphate 1.0g)
Dextrose (glucose 2.0g)
Bromthymol Blue (bromothymol blue 0.08g
Agar (agar 15.0g)
PH6.8 + 0.2 (25 C)