堿性膽鹽瓊脂干粉配制方法
發(fā)布時(shí)間：
2022-12-27
作者：
日水生物
堿性膽鹽瓊脂配制方法:
稱取本品47.5g于1L蒸餾水或去離子水中可按比例增加或減少配制量）,加熱煮沸至完全溶解,分裝,121℃高壓滅菌15分鐘,滅菌結(jié)束后請(qǐng)搖勻,以防瓊脂沉積于器皿底部而凝固，備用。
Formula(堿性膽鹽瓊脂配制方法配方)： g/L
Peptone 蛋白胨 20.0g
Beef Extract牛肉浸粉 5.0g
Sodium Chloride氯化鈉 5.0g
Bile Salts牛膽鹽 2.5g
Agar瓊脂 15.0g
pH8.4±0.1(25℃)
Alkaline salt agar preparation method:
Call this product 47.5g in 1L distilled water or deionized water to increase or reduce the amount of preparation), heat and boil to completely dissolve, pack, 121 degrees centigrade sterilization for 15 minutes, after sterilization, please shake well in order to prevent agar from depositing at the bottom of the utensils and solidified. Spare.
Formula (formula):g/L
Peptone peptone 20.0g
Beef Extract beef dipping powder 5.0g
Sodium Chloride sodium chloride 5.0g
Bile Salts bovine bile salt 2.5G
Agar agar 15.0g
PH8.4 + 0.1 (25 C)