志賀氏菌增菌肉湯基礎(chǔ)培養(yǎng)基配方
發(fā)布時(shí)間：
2022-12-27
作者：
日水生物
志賀氏菌增菌肉湯配制方法:
稱取本品31.5g于1L蒸餾水或去離子水中（可按比例增加或減少配制量）,加熱煮沸至完全溶解,分裝,121℃高壓滅菌15分鐘,冷卻至50-55℃,每225ml中添加1支20410,備用。
Formula(志賀氏菌增菌肉湯配方)： g/L
Tryptone （胰蛋白胨） 20.0g
Dextrose(葡萄糖) 1.0g
Dipotassium phosphate
(磷酸氫二鉀) 2.0g
Monopotassium phosphate
(磷酸二氫鉀) 2.0g
Sodium Chloride(氯化鈉) 5.0g
Tween 80(吐溫80) 1.5ml
pH7.0±0.2(25℃)
Preparation method:
According to take this product 31.5 g in 1 l of distilled water or deionized water (can be in proportion to increase or decrease preparation), heating boiling until completely dissolved, repackaging, 121 ℃ high pressure sterilization for 15 minutes, cooled to 50 to 55 ℃, each 225 ml, 1-20410 is added to the standby.
Formula: g/L
Tryptone (Tryptone) 20.0g
Dextrose(glucose) 1.0g
Dipotassium phosphate
(dipotassium hydrogen phosphate) 2.0g
Monopotassium phosphate
(potassium dihydrogen phosphate) 2.0g
Sodium Chloride (nacl) 5.0 g
Tween 80(twain 80) 1.5ml
PH7.0 + / - 0.2 (25 ℃)