LB培養(yǎng)基和MS培養(yǎng)基的比較
發(fā)布時(shí)間：
2022-11-16
作者：
LB培養(yǎng)基和MS培養(yǎng)基的比較
一、LB培養(yǎng)基
LB一般被解釋為Luria-Bertani培養(yǎng)基，根據(jù)其發(fā)明人貝爾塔尼（Giuseppe Bertani）的說法，這個(gè)名字來源于英語的lysogeny broth，即溶菌肉湯。1．用途
一般用該培養(yǎng)基來預(yù)培養(yǎng)菌種，使菌種成倍擴(kuò)增，達(dá)到使用要求，可分為液體培養(yǎng)基和固體培養(yǎng)基。
2．LB培養(yǎng)基的配方
胰蛋白胨（Tryptone)）10g/L
國產(chǎn)的胰蛋白胨一般是以新鮮牛肉和牛骨經(jīng)胰酶消化而得到，與進(jìn)口的成分和工藝不同。進(jìn)口的胰蛋白胨以酪蛋白為基礎(chǔ)進(jìn)行消化，實(shí)為胰酪蛋白胨；而國產(chǎn)的是以肉、骨為原料。含有豐富的氮源、氨基酸等，可配制各種微生物培養(yǎng)基，用于細(xì)菌的培養(yǎng)、分離、增殖、鑒定
酵母提取物（YE）5g/L
采用以蛋白質(zhì)含量豐富的食用酵母為原料，采用自溶、酶解、分離、濃縮等現(xiàn)代生物高新技術(shù)，將酵母細(xì)胞內(nèi)的蛋白質(zhì)、核酸等進(jìn)行降解后精制而成的一種棕黃色可溶性膏狀或淺黃色粉狀純天然制品。主要成分為多肽、氨基酸、呈味核苷酸、B族維生素及微量元素。
氯化鈉（NaCl）10g/L
另外根據(jù)經(jīng)驗(yàn)值用NaOH調(diào)節(jié)該培養(yǎng)基的pH，使其達(dá)到7.4，該pH適合目前使用最廣的原核表達(dá)菌種大腸桿菌（E.coli）的生長。
二、MS培養(yǎng)基
Murashige和Skoog于1962年為煙草細(xì)胞培養(yǎng)設(shè)計(jì)的，其特點(diǎn)是無機(jī)鹽和離子濃度較高，是較穩(wěn)定的離子平衡溶液，它的硝酸鹽含量高，其養(yǎng)分的數(shù)量和比例合適，能滿足植物細(xì)胞的營養(yǎng)和生理需要，因而適用范圍比較廣，多數(shù)植物組織培養(yǎng)快速繁殖用它作為培養(yǎng)基的基本培養(yǎng)基。 基于此，這種培養(yǎng)基就用他們的名字來命名了。
可用于誘導(dǎo)愈傷組織，也可用于胚、莖段、莖尖及花藥的培養(yǎng)，其液體培養(yǎng)基用于細(xì)胞懸浮培養(yǎng)時(shí)能獲得明顯的成功。
MS培養(yǎng)基的無機(jī)養(yǎng)分的數(shù)量和比例比較合適，足以滿足植物細(xì)胞在營養(yǎng)上和生理上的需要。和其它培養(yǎng)基的基本成分相比，MS培養(yǎng)基中的硝酸鹽、鉀和銨的含量高，這是它的顯著特點(diǎn)。
2．MS固體培養(yǎng)基的配方（單位：mg/L）
由大量元素、微量元素和有機(jī)成分組成。也可將大量元素、微量元素和有機(jī)成分分別配成濃度為配方的5-10倍，用時(shí)稀釋。
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Comparison of LB medium and MS medium
1. LB medium
LB is commonly interpreted as a luria-bertani culture medium, according to its inventor, Giuseppe Bertani, whose name comes from English lysogeny broth, or lysogeny broth.
1. use
In general, the medium is used to preculture the bacteria, which can be divided into liquid medium and solid medium, so that the bacteria can be multiplied multiple times and meet the usage requirements.
2. Recipe of LB medium
Tryptone 10g/L
Domestic tryptone is generally obtained from fresh beef and bovine bone through trypsin digestion, which is different from imported ingredients and techniques. The imported tryptone was digested on the basis of casein. And home - made meat, bone as raw materials. Rich in nitrogen sources, amino acids, etc., various microbial media can be prepared for bacterial culture, isolation, proliferation, identification
Yeast extract (YE) 5g/L
Based on protein content rich edible yeast as raw material, adopting autolyzed, enzymatic hydrolysis, separation, enrichment and other modern biological hi-tech, protein, nucleic acid in yeast cells, such as refined after degradation of soluble paste a tan or light yellow powder pure natural products. The main components are polypeptide, amino acid, flavor nucleotide, B vitamins and trace elements.
Sodium chloride (NaCl) 10g/L
In addition, according to the empirical value, the pH of the medium was adjusted with NaOH to reach 7.4, which is suitable for the growth of e. coli, the most widely used prokaryotic expression strain at present.
2. MS medium
Murashige and Skoog designed for tobacco cell culture in 1962, its characteristic is inorganic salt and ion concentration is higher, is a relatively stable ionic equilibrium solution, and its high nitrate content, its nutrient proportion and quantity of the right, to meet the nutrition and physical needs of plant cell, thus applicable scope is wide, the majority of plant tissue culture rapid propagation using it as the basic culture medium. Based on this, the culture medium was named after them.
1. use
It can be used to induce callus tissue, or for embryo, stem segment, stem tip and anther culture.
The amount and proportion of inorganic nutrients in MS medium are appropriate enough to meet the nutritional and physiological needs of plant cells. Compared with the basic components of other media, the content of nitrate, potassium and ammonium in MS medium is high, which is its remarkable feature.
2. Formulation of MS solid medium (mg/L)
Composed of a large number of elements, trace elements and organic components, the formula is as follows. A large number of elements, trace elements and organic components can also be mixed into 5-10 times the concentration of the formula respectively, which can be diluted in time.