DMEM培養(yǎng)基的成份有哪些？
發(fā)布時(shí)間：
2022-12-24
作者：
品牌/潔凈室微生物培養(yǎng)基
DMEM是 dulbecco's modified eagle medium的縮寫(xiě),是一種含各種氨基酸和葡萄糖的培養(yǎng)基，是在MEM培養(yǎng)基的基礎(chǔ)上研制的。與MEM比較增加了各種成分用量，同時(shí)又分為高糖型（低于4500mg/L）和低糖型(低于1000mg/L)。高糖型有利于細(xì)胞停泊于一個(gè)位置生長(zhǎng)，適于生長(zhǎng)較快、附著較困難腫瘤細(xì)胞等。所以其特點(diǎn)主要包括以下：
（1）氨基酸含量為依格爾培養(yǎng)基的2倍,且含有非必需氨基酸,如甘氨酸等；
（2）維生素含量為依格爾培養(yǎng)基的4倍；
（3）含有糖酵解途徑中的重要物質(zhì)——丙酮酸；
（4）含有微量的鐵離子.Eagle來(lái)自該培養(yǎng)基的發(fā)明人Harry Eagle
1640和DMEM培養(yǎng)基的區(qū)別
在組分和用途上有區(qū)別，主要是組分上的區(qū)別，查各自的配方表可知。用途上這兩種應(yīng)用都很廣泛，且都適合很多種細(xì)胞的培養(yǎng)。
RPMI 1640主要用于懸浮細(xì)胞培養(yǎng)?？蛇m用于哺乳動(dòng)物、特殊造血細(xì)胞、正?；驉盒栽錾陌准?xì)胞，雜交瘤細(xì)胞的培養(yǎng)。其它像K-562、HL-60、IM-9等成淋巴細(xì)胞、T細(xì)胞淋巴瘤細(xì)胞以及HCT-15上皮細(xì)胞等均可參考使用。
DMEM適用于貼壁細(xì)胞生長(zhǎng)，如各種實(shí)體瘤貼壁細(xì)胞。
DMEM培養(yǎng)基的成份：
含量mg/L
無(wú)水氯化鈣，200
硝酸鐵9H20，0.1
氯化鉀，400
無(wú)水硫酸二氫鈉，125
L鹽酸精氨酸，84
L鹽酸胱氨酸，63
L谷氨酸胺，584
L甘氨酸，30
L鹽酸組氨酸，42
L異亮氨酸，105
L亮氨酸，105
L甲硫氨酸，30
L苯丙氨酸，66
L絲氨酸，42
L蘇氨酸，95
L色氨酸，16
L酪氨酸鈉鹽，104
L泛酸鈣，4
氯化膽堿，4
葉酸，4
肌醇，7.2
核黃素，0.4
鹽酸硫胺，4
鹽酸吡哆辛，4
葡萄糖，4500
酚紅，15
The composition of DMEM medium
DMEM is the abbreviation of dulbecco's modified Eagle medium. It is a medium containing various amino acids and glucose. It is developed on the basis of MEM medium. Compared with MEM, the amount of various ingredients was increased, and it was also divided into high glucose (less than 4500mg/L) and low sugar type (less than 1000mg/L). High glucose is beneficial for cells to grow at a single location, suitable for rapid growth, difficult adhesion and tumor cells. So its characteristics include the following:
(1) the amino acid content is 2 times higher than the agar medium and contains non essential amino acids, such as glycine.
(2) vitamin content was 4 times higher than the agar medium.
(3) pyruvic acid, an important substance in the glycolysis pathway.
(4) trace iron ions.Eagle came from the inventor Harry Eagle of the medium.
The difference between 1640 and DMEM medium
1640 there is a difference between the medium and the DMEM medium in terms of components and uses, mainly on the difference between components. These two kinds of media are widely used and are suitable for the cultivation of many kinds of cells.
RPMI 1640 is mainly used for suspension cell culture. It can be used for the culture of hybridoma cells in mammals, hematopoietic cells, normal or malignant hyperplasia. Other cells such as K-562, HL-60, IM-9 and other lymphoblastic cells, T cell lymphoma cells and HCT-15 epithelial cells can be used for reference.
DMEM is suitable for adherent cell growth, such as various solid tumor adherent cells.
The components of the DMEM medium:
Content mg/L
Anhydrous calcium chloride, 200
Iron nitrate 9H20, 0.1
Potassium chloride, 400
Anhydrous sulphuric acid, two sodium hydrogen, 125
L arginine hydrochloride, 84
L cystine hydrochloride, 63
L glutamic acid amine, 584
L glycine, 30
L HCl histidine, 42
L isoleucine, 105
L leucine, 105
L methionine, 30
L phenylalanine, 66
L serine, 42
L threonine, 95
L tryptophan, 16
L tyrosine sodium salt, 104
L calcium pantothenate, 4
Choline chloride, 4
Folic acid, 4
Inositol, 7.2
Riboflavin, 0.4
Thiamine hydrochloride, 4
Pyridoxine hydrochloride, 4
（pyridoxine hydrochloride在培養(yǎng)基中起什么作用）吡哆醇鹽酸鹽：本品是機(jī)體內(nèi)很多酶如轉(zhuǎn)氨基酶、脫羧酶、消旋酶、脫氫酶、合成酶和羥化酶等的輔酶，且可幫助糖類(lèi)和蛋白質(zhì)的分解、利用。缺乏時(shí)有類(lèi)似煙酸的缺乏癥。此外維生素B6還參與色氨酸將煙酸轉(zhuǎn)化為5-羥色胺的反應(yīng)。并可刺激白細(xì)胞的生長(zhǎng)，是形成血紅蛋白所需要的物質(zhì)。
Glucose, 4500
Phenol red, 15