Eugon LT 100瓊脂培養(yǎng)基基礎(chǔ)配置方法
發(fā)布時(shí)間：
2022-12-27
作者：
Eugon LT 100瓊脂培養(yǎng)基基礎(chǔ)配制方法：稱取本品47.4g于1L蒸餾水或去離子水中，添加一支20204[吐溫-80]，微溫溶解,分裝,121℃高壓滅菌15分鐘,滅菌結(jié)束后請搖勻,以防瓊脂沉積于器皿底部而凝固,備用。
Eugon LT 100 Agar Medium Base Formula[配方]： g/L
Pancreatic Digest of Casein [胰酪蛋白胨]：15.0g
Enzymatic Digest of Soybean Meal[大豆蛋白胨] ： 5.0g
L-Cystine[L-胱氨酸]：0.7g
Sodium Chloride[氯化鈉]：4.0g
Sodium Sulfite[亞硫酸鈉]：0.2g
Dextrose[葡糖糖]：5.5g
Yolk Lecithin [卵黃卵磷脂]：1.0g
Octoxynol-9[Octoxynol-9] ：1.0g
Agar[瓊脂]：15.0g
pH7.0±0.2[25℃]
Eugon LT 100 agar medium basic preparation method: weigh 47.4g in 1L distilled water or deionized water, add a 20204 [Tween-80], slightly temperature dissolution, sub-package, 121 C high pressure sterilization for 15 minutes, after sterilization, shake well, in order to prevent agar deposited in the bottom of the vessel and solidify, reserve.
Eugon LT 100 Agar Medium Base Formula[formula]:g/L
Pancreatic Digest of Casein [casein peptone]:15.0g
Enzymatic Digest of Soybean Meal[soy peptone]:5.0g
L-Cystine[L- cystine]:0.7g
Sodium Chloride[NaCl]:4.0g
Sodium Sulfite[sodium sulfite]:0.2g
Dextrose[glucose]:5.5g
Yolk Lecithin [yolk lecithin]:1.0g
Octoxynol-9[Octoxynol-9]:1.0g
Agar[agar]:15.0g
PH7.0 + 0.2[25 C]