肉湯培養(yǎng)基D(水合乳糖肉湯)的配置
發(fā)布時(shí)間：
2022-12-27
作者：
山東培養(yǎng)基公司日水
肉湯培養(yǎng)基 D(水合乳糖肉湯)配制方法：稱取本品13.0g于1L蒸餾水或去離子水中(可按比例增加或減少配制量),加熱煮沸至完全溶解，121℃高壓滅菌15分鐘，滅菌結(jié)束后迅速冷卻，備用。（資料來自：青島日水生物官網(wǎng)）
配方：g/L
牛肉浸粉：3.0g
明膠蛋白胨：5.0g
一水乳糖：5.0g
pH6.9±0.2(25℃)
Broth culture medium D (hydrate Lactose Broth) preparation method: called this product 13.0g in the 1L distilled water or deionized water (can increase or reduce the amount of preparation), heat boiling to completely dissolved, 121 degrees centigrade sterilization for 15 minutes, after sterilization, quickly cooling, spare.
Formula: g/L
Beef dipping: 3.0g
Gelatin peptone: 5.0g
One water lactose: 5.0g
PH6.9 + 0.2 (25 C)