疊氮化鈉葡萄糖肉湯的配制方法
發(fā)布時(shí)間：
2022-12-27
作者：
日水生物
疊氮化鈉葡萄糖肉湯配制方法:
稱取本品34.7g于1L蒸餾水或去離子水中，加熱煮沸溶解，若配制雙料濃度的疊氮化鈉葡糖糖肉湯,干粉用量加倍，其他不變)，分裝,121℃高壓滅菌15分鐘,備用。
Formula疊氮化鈉葡萄糖肉湯配方： g/L
Tryptone 胰蛋白胨：15.0g
Sodium Chloride氯化鈉：7.5g
Beef Extract牛肉浸膏：4.5g
Dextrose葡萄糖：7.5g
Sodium Azide 疊氮化鈉：0.2g
pH7.2±0.225℃
Preparation method:
This product is called 34.7g in 1L distilled water or deionized water, heated and boiled to dissolve, if a double concentration of sodium azide glucose broth is prepared, the dosage of dry powder doubles, the other is constant, and is divided, sterilized at 121 C for 15 minutes, and spare.
Formula formula):g/L
Tryptone tryptone: 15.0g
Sodium Chloride sodium chloride: 7.5G
Beef Extract beef extract: 4.5g
Dextrose glucose: 7.5G
Sodium Azide sodium azide: 0.2g
PH7.2 + 0.225 C