3.5%氯化鈉三糖鐵瓊脂的配方
發(fā)布時間:
2022-12-27
作者:
日水生物
3.5%氯化鈉三糖鐵瓊脂的配方
配制方法:
稱取本品94.5g于1L蒸餾水或去離子水中,加熱煮沸至完全溶解,分裝試管,121℃高壓滅菌15分鐘,滅菌結(jié)束后制成高層斜面,備用。
Formula(配方):g/L
Peptone(蛋白胨) 15.0g
Proteose(月示蛋白胨) 5.0g
Beef Extract(牛肉浸出粉) 3.0g
Yeast Extract(酵母浸粉) 3.0g
Sodium Chloride(氯化鈉) 35.0g
Lactose(乳糖) 10.0g
Sucrose(蔗糖) 10.0g
Dextrose(葡萄糖) 1.0g
Ferrous Sulfate(硫酸亞鐵) 0.2g
Sodium Thiosulfate
(硫代硫酸鈉) 0.3g
Phenol Red(苯酚紅) 0.024g
Agar(瓊脂) 12.0g
pH7.4±0.2(25℃)
Formula of 3.5% sodium chloride trisaccharide ferroagar
Preparation method:
According to take this product 94.5 g in 1 l of distilled water or deionized water, heat to boil until completely dissolved, partial shipments in vitro, 121 ℃ high pressure sterilization for 15 minutes, after the sterilization cant made high-level, set aside.
Formula: g/L
Peptone (Peptone) 15.0g
Proteose (monthly peptone) 5.0g
Beef Extract 3.0g
Calcium Extract (Yeast Extract) 3.0g
Sodium Chloride (nacl) 35.0 g
Lactose (Lactose) 10.0g
Sucrose (Sucrose) 10.0g
Dextrose(glucose) 1.0g
Ferrous Sulfate (Ferrous Sulfate) 0.2g
Sodium Thiosulfate
(sodium thiosulphate) 0.3g
Phenol Red (Phenol Red) 0.024g
Agar (Agar) 12.0g
PH7.4 slightly + / - 0.2 (25 ℃)