氯化鈉三糖鐵瓊脂配方表
發(fā)布時間：
2022-12-27
作者：
日水生物
氯化鈉三糖鐵瓊脂配配制方法:
稱取本品74.3g于1L蒸餾水或去離子水中（可按比例增加或減少配制量）,加熱煮沸至完全溶解，分裝，121℃高壓滅菌15分鐘,滅菌結(jié)束后請搖勻，以防瓊脂沉積于器皿底部而凝固，備用。
Formula(配方)： g/L
Peptone （蛋白胨） 20.0g
Beef Extract（牛肉浸膏） 3.0g
Yeast Extract（酵母浸膏） 3.0g
Sodium Chloride(氯化鈉) 10.0g
Lactose（乳糖） 10.0g
Sucrose（蔗糖） 10.0g
Ferric citrate（檸檬酸鐵） 0.3g
Phenol Red（酚紅） 0.024g
Agar（瓊脂） 18.0g
pH7.4±0.2(25℃)
Product description
Preparation method:
According to take this product 74.3 g in 1 l of distilled water or deionized water (can be in proportion to increase or decrease preparation), heating boiling until completely dissolved, repackaging, 121 ℃ high pressure sterilization for 15 minutes, please shake after sterilization, to prevent AGAR deposited on the bottom of the vessel and solidification, and set aside.
Formula: g/L
Peptone (Peptone) 20.0g
Beef Extract 3.0g
Calcium Extract (Yeast Extract) 3.0g
Sodium Chloride (nacl) 10.0 g
Lactose (Lactose) 10.0g
Sucrose (Sucrose) 10.0g
Ferric citrate (Ferric citrate) 0.3g
Phenol Red (Phenol Red) 0.024g
Agar (Agar) 18.0g
PH7.4 slightly + / - 0.2 (25 ℃)