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氯化鈉營養(yǎng)瓊脂配制

發(fā)布時間:

2022-12-27

作者:

日水生物


氯化鈉營養(yǎng)瓊脂配方
 
氯化鈉營養(yǎng)瓊脂配方配制方法:
稱取本品36.0g于1L蒸餾水或去離子水中(可按比例增加或減少配制量),加熱煮沸至完全溶解,分裝,121℃高壓滅菌15分鐘,滅菌結(jié)束后請搖勻,以防瓊脂沉積于器皿底部而凝固,備用。
 
Formula(氯化鈉營養(yǎng)瓊脂配方): g/L
Peptone(蛋白胨)                                   3.0g
Beef Extract(牛肉浸粉)                        5.0g
Sodium Chloride(氯化鈉)                   10.0g
Agar(瓊脂)                                            18.0g
pH7.2±0.2(25℃)
 
 

Preparation method:
According to take this product 36.0 g in 1 l of distilled water or deionized water (can be in proportion to increase or decrease preparation), heating boiling until completely dissolved, repackaging, 121 ℃ high pressure sterilization for 15 minutes, please shake after sterilization, to prevent AGAR deposited on the bottom of the vessel and solidification, and set aside.
 
Formula: g/L
Peptone (Peptone) 3.0g
Beef Extract 5.0g
Sodium Chloride (nacl) 10.0 g
Agar (Agar) 18.0g
PH7.2 + / - 0.2 (25 ℃)