BBL 瓊脂培養(yǎng)基配方
發(fā)布時(shí)間：
2022-12-27
作者：
BBL 瓊脂培養(yǎng)基英文名稱：BBL Agar Medium
產(chǎn)品編號(hào)：11301
產(chǎn)品規(guī)格：250g/瓶
產(chǎn)品用途：用于雙歧桿菌分離培養(yǎng)
Formula(配方)： g/L
Peptone（蛋白胨） 15.0g
Yeast Extract (酵母浸粉) 2.0g
Dextrose(葡萄糖) 20.0g
Soluble Starch（可溶性淀粉） 0.5g
Sodium Chloride（氯化鈉） 5.0g
Tomato immersion powder（番茄浸粉） 5.0g
Tween 80（吐溫80） 1.0g
Ｌiver meal（肝粉） 0.3g
L-Cysteine（半胱氨酸） 0.5g
Agar(瓊脂) 15.0g
pH6.8±0.2 (25℃)
配制方法:
稱取本品64.3g于1L蒸餾水或去離子水 中,加熱煮沸至完全溶解，分裝，121℃高壓滅菌15分鐘，滅菌結(jié)束后請(qǐng)搖勻，以防瓊脂沉積于器皿底部而凝固，備用。
BBL Agar Medium
Product number: 11301
Product specification: 250g/ bottle
Application: for isolation and culture of bifidobacteria
Formula: g/L
Peptone (Peptone) 15.0g
Calcium Extract (Yeast Extract) 2.0g
Dextrose(glucose) 20.0g
Soluble Starch (Soluble Starch) 0.5 g
Sodium Chloride (nacl) 5.0 g
Tomato immersion powder (Tomato immersion powder) 5.0g
Tween 80 (twain 80) 1.0g
Liver meal 0.3g
L-cysteine (Cysteine) 0.5g
Agar(Agar) 15.0g
PH6.8 + / - 0.2 (25 ℃)
Preparation method:
According to take this product 64.3 g in 1 l of distilled water or deionized water, heat to boil until completely dissolved, repackaging, 121 ℃ high pressure sterilization for 15 minutes, please shake after sterilization, to prevent AGAR deposited on the bottom of the vessel and solidification, and set aside.