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Muller -Kauffmann四硫磺酸鈉肉湯基礎(chǔ)成分說(shuō)明

發(fā)布時(shí)間:

2022-12-27

作者:

日水生物


Muller -Kauffmann 四硫磺酸鈉肉湯基礎(chǔ)配制方法:稱(chēng)取本品105.8g于1L蒸餾水或去離子水中,使用鹽酸調(diào)節(jié)pH至7.0±0.2(25℃)加熱煮沸1分鐘,冷至25℃,每100ml中加入20315、20316各一支,混勻,分裝。
 
Formula(配方): g/L
 
Peptone\蛋白胨:10.0g
Beef Extract\牛肉浸粉:5.0g
Sodium Chloride\氯化鈉:3.0g
Oxgall\牛膽汁:4.7g
Calcium Carbonate\碳酸鈣:45.0g
Sodium Thiosulfate\硫代硫酸鈉:38.1g
Muller -Kauffmann four sodium sulfonic acid sodium broth preparation method: called the product 105.8g in 1L distilled water or deionized water, using hydrochloric acid to adjust pH to 7 + 0.2 (25) heating boil for 1 minutes, cold to 25 degrees, each 100ml added 20315, 20316, mix, separate.
Formula (formula):g/L
Peptone: 10.0g
Beef dipping: 5.0g
Sodium chloride: 3.0g
Bovine bile: 4.7G
Calcium carbonate: 45.0g
Sodium thiosulfate: 38.1g