配置macconkey agar medium麥康凱瓊脂培養(yǎng)基方法
發(fā)布時(shí)間：
2022-12-27
作者：
山東培養(yǎng)基公司日水生物
macconkey agar medium麥康凱瓊脂培養(yǎng)基配制方法：稱取本品54.0g于1L蒸餾水或去離子水中（可按比例增加或減少配制量），加熱煮沸至完全溶解,分裝,121℃高壓滅菌15分鐘,滅菌結(jié)束后請(qǐng)搖勻，以防瓊脂沉積于器皿底部而凝固，備用。（資料來(lái)自：青島日水生物官網(wǎng)）
配方： g/L
胨：20.0g
乳糖：10.0g
氯化鈉：5.0g
牛膽鹽：5.0g
中性紅：0.03g
瓊脂：14.0g
pH7.2±0.2(25℃)
MacConkey agar medium wheat Kang Kai agar medium preparation method: call this product 54.0g in 1L distilled water or deionized water (can increase or reduce the dosage in proportion), heat and boil to completely dissolve, pack, 121 degrees centigrade sterilization for 15 minutes, after sterilization, please shake well in order to prevent agar from depositing at the bottom of the utensils and solidified. Spare.
Formula: g/L
Peptone: 20.0g
Lactose: 10.0g
Sodium chloride: 5.0g
Bovine bile salt: 5.0g
Neutral red: 0.03g
Agar: 14.0g
PH7.2 + 0.2 (25 C)