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麥康凱瓊脂培養(yǎng)基的配制方法

發(fā)布時間:

2022-12-27

作者:


麥康凱瓊脂培養(yǎng)基

 

英文名稱:MacConkey  Agar
產(chǎn)品編號:12721-1
產(chǎn)品規(guī)格:250g/瓶
產(chǎn)品用途:用于大腸埃希氏菌的選擇性分離
 
Formula(配方): g/L
Gelatin of trypsin digestion content(明膠胰酶水解物)    17.0g
Peptone(胨)               3.0g                          
Lactose(乳糖)             10.0g                         
Sodium Chloride(氯化鈉)        5.0g                    
Sodium deoxycholate(脫氧膽酸鈉)      1.5g                         
Neutral Red(中性紅)      0.03g
Crystal Violet(結(jié)晶紫)    0.001g               
Agar(瓊脂)                   13.5g                          
pH7.1±0.2(25℃)
 
日水培養(yǎng)基的配制方法:
    稱取本品50.0g于1L蒸餾水或去離子水中,加熱煮沸至完全溶解,分裝,121℃高
壓滅菌15分鐘,滅菌結(jié)束后請搖勻,以防瓊脂沉積于器皿底部而凝固,備用。

 


 

McConkey AGAR medium

 
 
 
MacConkey Agar
Product number: 12721-1
Product specification: 250g/ bottle
Application: for selective isolation of escherichia coli
 
Formula: g/L
Gelatin of trypsin digestion content (Gelatin hydrolysate pancreatic enzyme) 17.0 g
Peptone (Peptone) 3.0g
Lactose (Lactose) 10.0g
Sodium Chloride (nacl) 5.0 g
Sodium deoxycholate is 1.5g
Neutral Red is 0.03g Neutral
Crystal Violet 0.001g
Agar (Agar) 13.5g
PH7.1 + / - 0.2 (25 ℃)
 
Preparation method:
According to take this product 50.0 g in 1 l of distilled water or deionized water, heating boiling until completely dissolved, repackaging, 121 ℃ high
Press sterilization for 15 minutes, shake well after sterilization, to prevent agar-agar deposition at the bottom of the container and solidify, set aside.