麥康凱瓊脂基礎(chǔ)培養(yǎng)基的配方(macconkey agar base)
發(fā)布時間：
2022-12-27
作者：
日水生物
麥康凱瓊脂基礎(chǔ)培養(yǎng)基的配制方法: 稱取本品40.0g于1L蒸餾水或去離子水中/可按比例增加或減少配制量），加熱煮沸至完全溶解，分裝，121℃高壓滅菌15分鐘，滅菌結(jié)束后請搖勻，以防瓊脂沉積于器皿底部而凝固,備用。可直接使用或添加0.1%的單一或復(fù)合碳源。
Formula/配方： g/L
Peptone/蛋白胨：17.0g
Proteose/示蛋白胨：3.0g
Sodium Chloride/氯化鈉：5.0g
Bile Salts No.3/3號膽鹽：1.5g
Neutral Red/中性紅：0.03g
Crystal Violet/結(jié)晶紫：0.001g
Agar/瓊脂：13.5g
pH7.1±0.2/25℃
Preparation method: call this product 40.0g in 1L distilled water or deionized water / can increase or reduce the dosage in proportion), heat and boil to completely dissolve, pack, sterilize at 121 centigrade for 15 minutes, after sterilization, please shake well in order to prevent agar from depositing at the bottom of the utensils. A single or compound carbon source can be directly used or added to 0.1%.
Formula: g/L
Peptone: 17.0g
Peptone: 3.0g
Sodium chloride: 5.0g
No. 3 bile salt: 1.5g
Neutral red: 0.03g
Crystal violet: 0.001g
Agar/ agar: 13.5g
PH7.1 + 0.2/25 C