麥康凱瓊脂的配方是什么？
發(fā)布時(shí)間：
2022-12-27
作者：
日水生物
麥康凱瓊脂的配制方法:
稱取本品57.0g于1L蒸餾水或去離子水中可按比例增加或減少配制量）,加熱煮沸至完全溶解,分裝,121℃高壓滅菌15分鐘,滅菌結(jié)束后請(qǐng)搖勻,以防瓊脂沉積于器皿底部而凝固，備用。
Formula配方： g/L
Peptone蛋白胨：20.0g
Lactose乳糖：10.0g
Bile Salts膽鹽：5.0g
Sodium Chloride氯化鈉：5.0g
Neutral Red中性紅：0.025g
Crystal Violet結(jié)晶紫：0.001g
Agar瓊脂：17.0g
pH7.2±0.2(25℃)
Preparation method:
Call this product 57.0g in 1L distilled water or deionized water to increase or reduce the amount of preparation), heat and boil to completely dissolve, pack, 121 degrees centigrade sterilization for 15 minutes, after sterilization, please shake well in order to prevent agar from depositing at the bottom of the utensils and solidified. Spare.
Formula formula: g/L
Peptone peptone: 20.0g
Lactose lactose: 10.0g
Bile Salts bile salt: 5.0g
Sodium Chloride sodium chloride: 5.0g
Neutral Red neutral red: 0.025g
Crystal Violet crystal violet: 0.001g
Agar agar: 17.0g
PH7.2 + 0.2 (25 C)