麥康凱瓊脂的配方是什么？
發(fā)布時(shí)間：
2022-12-27
作者：
日水生物
麥康凱瓊脂配制方法:稱取本品50.0g于1L蒸餾水或去離子水中，加熱煮沸至完全溶解，分裝，121℃高壓滅菌15分鐘,滅菌結(jié)束后請(qǐng)搖勻，以防瓊脂沉積于器皿底部而凝固，備用。資料來(lái)自日水生物
Formula(麥康凱瓊脂配方)： g/L
Pancreatic Digest of Casein\胰酪蛋白胨：1.5g
Pancreatic Digest of Gelatin\明膠蛋白胨：17.0g
Peptic Digest of Animal Tissue\動(dòng)物組織消化物：1.5g
Lactose\乳糖：10.0g
Bile Salt\牛膽鹽：1.5g
Sodium Chloride\氯化鈉：5.0g
Crystal Violet\結(jié)晶紫：0.001g
Neutral Red\中性紅：0.03g
Agar\瓊脂：13.5g
pH7.1±0.2(25℃)
Mae Kang Kai agar preparation method: call this product 50.0g in 1L distilled water or deionized water, heat and boil to completely dissolve, pack, 121 centigrade high pressure sterilization for 15 minutes, after sterilization, please shake well in order to prevent agar from depositing at the bottom of the vessel and solidified, spare. Data from diurnal aquatic organisms
Formula (formula):g/L
Casein peptone: 1.5g
Gelatin peptone: 17.0g
Animal tissue digestion: 1.5g
Lactose: 10.0g
Bovine bile salt: 1.5g
Sodium chloride: 5.0g
Crystal violet: 0.001g
Neutral red: 0.03g
Agar: 13.5g
PH7.1 + 0.2 (25 C)