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制備膽鹽硫乳瓊脂培養(yǎng)基dhl方法

發(fā)布時(shí)間:

2022-12-27

作者:

日水生物


膽鹽硫乳瓊脂培養(yǎng)基DHL配制方法:稱取本品64.3g于1L蒸餾水或去離子水中,攪拌煮沸溶解1分鐘,冷卻至50—55℃
傾注平板。
 
配方: g/L
 
蛋白胨:20.0g
牛肉浸出粉:3.0g
蔗糖:10.0g
乳糖:10.0g
去氧膽酸鈉:1.0g
硫代硫酸鈉:2.3g
檸檬酸鈉:1.0g
檸檬酸鐵按:1.0g
中性紅:0.03g
瓊脂:16.0g
pH7.2±0.1(25℃)
Preparation method of bile salt sulfur milk agar DHL: the product is called 64.3g in 1L distilled water or deionized water, stirring and boiling for 1 minutes, cooling to 50 to 55 degrees centigrade.
Pour the flat plate.
Formula: g/L
Peptone: 20.0g
Beef extract powder: 3.0g
Sucrose: 10.0g
Lactose: 10.0g
Sodium deoxycholate: 1.0g
Sodium thiosulfate: 2.3g
Sodium citrate: 1.0g
Iron citrate: 1.0g
Neutral red: 0.03g
Agar: 16.0g
PH7.2 + 0.1 (25 C)