Skirrow 瓊脂基礎(chǔ)培養(yǎng)基配方
發(fā)布時(shí)間：
2022-12-27
作者：
日水生物
Skirrow 瓊脂基礎(chǔ)培養(yǎng)基配制方法:
稱取本品4.25g于100ml蒸餾水或去離子水中，加熱煮沸至完全溶解，分裝，121℃高壓滅菌15分鐘,冷至50-55℃，添加1支20702【彎曲菌分離瓊脂添加劑(頭孢哌酮、兩性霉
素B、利福平）】和1支20703（FBP溶液），若添加一支20712（改良Skirrow瓊脂基礎(chǔ)添加劑）和7ml無菌脫纖維羊血，則組成改良Skirrow瓊脂，混勻，備用。
Formula(Skirrow 瓊脂基礎(chǔ)培養(yǎng)基配方)： g/L
Peptone(蛋白胨) 15.0g
Tryptone (胰蛋白胨) 2.5g
Yeast Extract(酵母浸粉) 5.0g
Sodium Chloride(氯化鈉) 5.0g
Agar(瓊脂) 15.0g
pH7.2±0.2(25℃)
Preparation method:
Said the 4.25 g amount of this product in 100 ml of distilled water or deionized water, heat to boil until completely dissolved, repackaging, 121 ℃ high pressure sterilization for 15 minutes, cold to 50 to 55 ℃, 1-20702 [bending bacteria separation AGAR additives (cefoperazone, amphoteric mildew
B, rifampicin) 】 1 20703 place (FBP) solution, and if you add a 20712 (improved Skirrow AGAR base additives) and 7 ml sterile fibre sheep blood, comprise modified Skirrow AGAR, blending, and set aside.
Formula: g/L
Peptone(Peptone) 15.0g
Tryptone (Tryptone) 2.5g
Calcium Extract(Yeast Extract) 5.0g
Sodium Chloride (nacl) 5.0 g
Agar(Agar) 15.0g
PH7.2 + / - 0.2 (25 ℃)